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Class 12 Biotechnology and its Applications worksheet
CBSE Biology. Bt crops and cry genes, RNAi, recombinant insulin, gene therapy, transgenic animals, ethics. Every sheet has 20 questions in three sections and an answer key, and the numbers change every time.
Sample questions
Real questions from this chapter, as the app sets them. Tap Answer to see the answer and working.
Section A · multiple choice
- 1.
Bt toxin is produced by:
- Escherichia coli
- Thermus aquaticus
- Agrobacterium tumefaciens
- Bacillus thuringiensis
Answer
(d) Bacillus thuringiensis
- 2.
Which of these is a transgenic cow (1997) whose milk contains human α-lactalbumin?
- Rosie
- Biopiracy
- GEAC
- ELISA
Answer
(a) Rosie
- 3.
The Bt toxin becomes active in the insect gut because of:
- the acidic pH
- digestive lipases
- the low temperature
- the alkaline pH
Answer
(d) the alkaline pH
Section B · short answer
- 4.
What are GMOs? Give two benefits of GM crops.
Answer
Plants, bacteria, fungi or animals whose genes have been altered by manipulation. GM crops tolerate abiotic stresses, need fewer chemical pesticides, lose less after harvest, use minerals better and can have better nutritional value (any two).
- 5.
How many polypeptide chains, amino acids and disulphide bridges are there in 3 molecules of mature human insulin? What part of proinsulin is removed?
Answer
6 chains, 153 amino acids, 9 disulphide bridges; the C peptide
A chain 21 + B chain 30 amino acids; two bridges between the chains and one within A
Section C · long answer
- 6.
Explain how Bt cotton is made pest-resistant.
Answer
Bacillus thuringiensis forms crystal proteins that kill certain insects. The protein exists as an inactive protoxin; in the alkaline gut of an insect that eats it, the crystals dissolve and the activated toxin binds to midgut epithelial cells, creating pores that make them swell and burst, killing the insect. The cry genes cryIAc and cryIIAb, which control cotton bollworms, have been put into cotton; cryIAb controls the corn borer.
Fresh sheets for your child
In the app, every Biotechnology and its Applications sheet is new: the app remembers the questions your child has already had and leaves them out. Print the sheet as a PDF, or use notebook mode and write the answers in a notebook.
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